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Living Modified Organism (LMO)
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BCH-LMO-SCBD-110940-2   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 27 Sep 2016 last updated: 06 Apr 2023
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Oilseed rape modified for increased myristic acid content in the seeds
EN
NBM99-ClFatB4 x NAT4; NBM99-EnClFatB4 x NAT4
No
A stacked oilseed rape (Brassica napus) was produced by crossing modified parental lines to produce myristic acid (C14:0; up to 10%, not normally present) and increase the levels of palmitic acid (C16:0) in the oil. The modified oil seed rape expresses Escherichia coli 1-acyl-sn-glycerol-3-phosphate acyltransferase, which catalyses the linkage of medium-chain fatty acids to the sn-2 position of lysophosphatidic acid, and Cuphea lanceolata acyl-[ACP] thioesterase, which is involved in in chain termination during de novo fatty acid synthesis with myristyl- and palmityl-[ACP] acting as substrates. The modified oilseed rape additionally expresses E. coli neomycin phosphotransferase II, which was used as an antibiotic selectable marker during transformation. 
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
  • BCH-LMO-SCBD-110939-1 Living Modified Organism Brassica napus modified for altered fatty acid metabolism
    Deutsche Saatveredelung AG(DSV) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-LMO-SCBD-101385-4 Living Modified Organism Brassica napus modified for altered fatty acid metabolism
    Bundesanstalt für Züchtungsforschung an Kulturpflanzen(BAZ) | Changes in quality and/or metabolite content (Lipid and fatty acids), Resistance to antibiotics (Kanamycin)
  • BCH-ORGA-SCBD-12083-7 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Crops
EN
Characteristics of the modification process
pNAT4, and pNBM99-ClFatB4 or pNBM99-ENClFatB4
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-103920-2 Acyl-acyl carrier protein thioesterase promoter | Cuphea lanceolata (Cigar Flower)
    Promoter
  • BCH-GENE-SCBD-101362-5 Acyl-acyl carrier protein thioesterase | Cuphea lanceolata (Cigar Flower)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-103921-2 Acyl-acyl carrier protein thioesterase terminator | Cuphea lanceolata (Cigar Flower)
    Terminator
  • BCH-GENE-SCBD-101521-6 Napin gene promoter | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Promoter
  • BCH-GENE-SCBD-110888-1 1-acyl-sn-glycerol-3-phosphate acyltransferase coding sequence | Escherichia coli (ECOLX)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
  • BCH-GENE-SCBD-110908-1 Napin gene terminator | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)
    Leader
  • BCH-GENE-SCBD-110890-1 1-acyl-sn-glycerol-3-phosphate acyltransferase coding sequence | Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)
    Protein coding sequence | Changes in quality and/or metabolite content (Lipid and fatty acids)
DNA insert from vector pNAT4
The parental genome contains two gene cassettes: Escherichia coli 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC) and neomycin phosphotransferase II (nptII).

The plsC coding sequence is under control of a Brassica napus napin promoter and terminator. Due to the seed-specific nature of the promoter, plsC is expected to be expressed only in seed tissues.

The nptII coding sequence is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and terminator. Due to the nature of the promoter, high-levels of constitutive expression in all plant tissues is expected.

DNA insert from vector pNBM99-ClFatB4 and pNBM99-EnClFatB4
The parental genome contains two gene cassettes: Cuphea lanceolata acyl-[ACP] thioesterase (fatB4) and nptII.

The fatB4 coding sequence is under control of its own (C. lanceolata fatB4) promoter and terminator. Due to the nature of the promoter, seed-specific expression is expected.

The nptII coding sequence is under control of a CaMV 35S promoter and terminator. Due to the nature of the promoter, high-levels of constitutive expression in all plant tissues is expected.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
EN

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